Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues.

doi: 10.1369/0022155416641028

Figure Lengend Snippet: Figure 1. Protein expression of AQP9 in normal tissues. (A) High membranous expression was observed in hepatocytes. (B) Medium level cytoplasmic expression was found in a subset of lymphoid cells in appendix (a, b). In bone marrow (C) and cells in red pulp of spleen (D), a subset of cells showed medium level cytoplasmic expression (c-f). (E) Moderate AQP9 immunoreactivity was also observed in cytoplasm of a subset of non-germinal center cells in tonsil (g, h). (F) In kidney, endothelial cells of renal glomeruli (i) displayed medium level expression, whereas renal tubules (j) showed weak expression. Scale, 100 µm.

Article Snippet: Anti-AQP9 antibodies sc-28623 and sc-74409 (Santa Cruz Biotechnology), as well as two internally generated antibodies were also tested on the immunohistochemistry platform but were evaluated as not suitable due to nonspecific staining and inconsistency with RNA-seq data (Supplementary Fig. 1).

Techniques: Expressing

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues.

doi: 10.1369/0022155416641028

Figure Lengend Snippet: Figure 2. Protein expression of AQP9 in cancer tissues. High expression was observed in the membranes of tumor cells in hepatocel- lular carcinomas (A), whereas no expression was found in cholangiocarcinomas (B). Moreover, a high to medium level of membranous expression was noted in single cases of renal cancer (C) and urothelial cancer (D). Scale, 100 µm.

Article Snippet: Anti-AQP9 antibodies sc-28623 and sc-74409 (Santa Cruz Biotechnology), as well as two internally generated antibodies were also tested on the immunohistochemistry platform but were evaluated as not suitable due to nonspecific staining and inconsistency with RNA-seq data (Supplementary Fig. 1).

Techniques: Expressing

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues.

doi: 10.1369/0022155416641028

Figure Lengend Snippet: Figure 3. Protein expression of AQP9 in synovial rheumatoid arthritis patients. Low cytoplasmic expression was observed in syno- vial lining (a) and mesenchymal cells (b) of two patients before Humira treatment (A and C). In one patient (B), the expression in synovial lining (c) was lost after Humira treatment, whereas the cytoplasmic expression was still observed in mesenchymal cells (d). In the other patient (D), the expression was unaltered in synovial lining (e), while no expression was found in mesenchymal cells (f). Scale, 100 µm.

Article Snippet: Anti-AQP9 antibodies sc-28623 and sc-74409 (Santa Cruz Biotechnology), as well as two internally generated antibodies were also tested on the immunohistochemistry platform but were evaluated as not suitable due to nonspecific staining and inconsistency with RNA-seq data (Supplementary Fig. 1).

Techniques: Expressing

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues.

doi: 10.1369/0022155416641028

Figure Lengend Snippet: Figure 4. Protein expression of AQP9 in cell lines. (A) High expression was observed in membranes of LP-1 myeloma cells. Medium level expression in a subset of cells was found in U266/70 cells (B), whereas no or only very low expression was noted in U-87 MG cells (C) and HepG2 cells (D). Scale, 100 µm.

Article Snippet: Anti-AQP9 antibodies sc-28623 and sc-74409 (Santa Cruz Biotechnology), as well as two internally generated antibodies were also tested on the immunohistochemistry platform but were evaluated as not suitable due to nonspecific staining and inconsistency with RNA-seq data (Supplementary Fig. 1).

Techniques: Expressing